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Biotinylated Human LILRB5/CD85c/LIR-8 Protein (LIL-HM4B5B) Summary
Product Description |
Recombinant Biotinylated Human LILRB5/CD85c/LIR-8 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg18-His456. |
Product Background |
LILRB5, also known as CD85c andLIR-8, belongs to a family of transmembrane glycoproteins that negatively regulate immune cell activation. Mature human LIR-8 consists of a 435 amino acid (aa) extracellular domain with four Ig-like domains, a 21 aa transmembrane segment, and a 111 aa cytoplasmic domain with two immunoreceptor tyrosine-based inhibitory motifs (ITIM). LILRB5 may act as receptor for class I MHC antigens. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Biotinylated Human LILRB5/CD85c/LIR-8 Protein (LIL-HM4B5B) Specifications/Details
Protein |
LILRB5/CD85c/LIR-8 |
Synonyms |
CD85C ; LIR-8; LIR8 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Arg18-His456 |
Molecular Weight |
The protein has a predicted MW of 49.9 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Biotinylated Human LILRB5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Biotinylated Human LILRB5 is greater than 95% as determined by SEC-HPLC. |
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