Product Description |
Recombinant Biotinylated Human Mature TGF beta 2 Protein is expressed from HEK293 with Avi tag at the C-Terminus.It contains Ala303-Ser414. |
Product Background |
Transforming growth factor beta(2) (TGF-beta(2)), a growth regulator of human lens epithelial cells (HLECs), also regulates the death of these cells. TGF-beta(2)-induced apoptosis in HLECs was preceded by an induction of reactive oxygen species (ROS) and a decrease in glutathione in the intracellular content, indicating that this factor induces oxidative stress in HLECs. |
Product Category |
Recombinant Protein |
Target |
Mature TGF beta 2 |
Synonyms |
TGFB2; TGFBR2; TGFR2; TbetaR-II; TGFβR2; BB105277; TGF-beta 2; TβR-II; TGF-β 2 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ala303-Ser414 |
Molecular Weight |
The protein has a predicted MW of 14.53 kDa same as Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human Mature TGF beta 2, Avi Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 0.17ug/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 4mM HCl. Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 4mM HCl. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Biotinylated Human Mature TGF beta 2, Avi Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 0.17ug/ml determined by ELISA (QC Test). |
|
|
Biotinylated Human Mature TGF beta 2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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