Biotinylated Human Mature TGF beta 2 Protein (TG2-HM00MB)

  • Express System: HEK293
  • Product Tag: C-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human Mature TGF beta 2, Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 0.27ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ala303-Ser414

Catalog: TG2-HM00MB

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Product Description Recombinant Biotinylated Human Mature TGF beta 2 Protein is expressed from HEK293 with Avi tag at the C-Terminus.It contains Ala303-Ser414.

Product Background

 Transforming growth factor beta(2) (TGF-beta(2)), a growth regulator of human lens epithelial cells (HLECs), also regulates the death of these cells. TGF-beta(2)-induced apoptosis in HLECs was preceded by an induction of reactive oxygen species (ROS) and a decrease in glutathione in the intracellular content, indicating that this factor induces oxidative stress in HLECs.

Product Category

 Recombinant Protein

Target

 Mature TGF beta 2

Gene/Protein ID

 P61812-1

Species

 Human

Exact Sequence

 Ala303-Ser414
Molecular Weight The protein has a predicted MW of 14.53 kDa same as Tris-Bis PAGE result.

Activity

 Immobilized Biotinylated Human Mature TGF beta 2, Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 0.27ug/ml determined by ELISA. See testing image for detail.

Product Tag

 C-Avi

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 4mM HCl. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 4mM HCl.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human Mature TGF beta 2, Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 0.27ug/ml determined by ELISA.
Biotinylated Human Mature TGF beta 2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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