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Biotinylated Human P-Selectin/CD62P Protein (SEL-HM40PB) Summary
Product Description |
Recombinant Biotinylated Human P-Selectin/CD62P Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Trp42-Ala771. |
Product Background |
Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Biotinylated Human P-Selectin/CD62P Protein (SEL-HM40PB) Specifications/Details
Protein |
P-Selectin/CD62P |
Synonyms |
FLJ45155; GMP140; GRMP; PADGEM; PSEL; P-Selectin; SELP; CD62P; CD62; LECAM3 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Trp42-Ala771 |
Molecular Weight |
The protein has a predicted MW of 82.9 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human P-Selectin, His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-P-Selectin Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test). |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% saccharose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human P-Selectin, His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-P-Selectin Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test). |
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Biotinylated Human P-Selectin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human P-Selectin is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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