Biotinylated Human P-Selectin/CD62P Protein (SEL-HM40PB)
Biotinylated Human P-Selectin/CD62P Protein (SEL-HM40PB)
Biotinylated Human P-Selectin/CD62P Protein (SEL-HM40PB)
Biotinylated Human P-Selectin/CD62P Protein (SEL-HM40PB)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human P-Selectin, His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-P-Selectin Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Trp42-Ala771
Catalog #SEL-HM40PB
Regular price $577.50
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Product Description

Recombinant Biotinylated Human P-Selectin/CD62P Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Trp42-Ala771.

Product Background

Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

P-Selectin/CD62P

Synonyms

FLJ45155; GMP140; GRMP; PADGEM; PSEL; P-Selectin; SELP; CD62P; CD62; LECAM3

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Trp42-Ala771

Molecular Weight

The protein has a predicted MW of 82.9 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Human P-Selectin, His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-P-Selectin Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% saccharose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human P-Selectin, His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-P-Selectin Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human P-Selectin, His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-P-Selectin Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test).
Biotinylated Human P-Selectin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human P-Selectin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human P-Selectin is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human P-Selectin is greater than 95% as determined by SEC-HPLC.

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