Biotinylated Human P-Selectin/CD62P Protein (SEL-HM40PB)
Biotinylated Human P-Selectin/CD62P Protein (SEL-HM40PB)
Biotinylated Human P-Selectin/CD62P Protein (SEL-HM40PB)
Biotinylated Human P-Selectin/CD62P Protein (SEL-HM40PB)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human P-Selectin, His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-P-Selectin Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Trp42-Ala771
Catalog #SEL-HM40PB
Regular price $525.00
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Product Description

Recombinant Biotinylated Human P-Selectin/CD62P Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Trp42-Ala771.

Product Background

Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation.

Product Category

Recombinant Protein

Protein

P-Selectin/CD62P

Synonyms

FLJ45155; GMP140; GRMP; PADGEM; PSEL; P-Selectin; SELP; CD62P; CD62; LECAM3

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Trp42-Ala771

Molecular Weight

The protein has a predicted MW of 82.9 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Human P-Selectin, His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-P-Selectin Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% saccharose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human P-Selectin, His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-P-Selectin Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human P-Selectin, His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-P-Selectin Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test).
Biotinylated Human P-Selectin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human P-Selectin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human P-Selectin is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human P-Selectin is greater than 95% as determined by SEC-HPLC.

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