Biotinylated Human TIGIT Protein (TIG-HM410B)
Biotinylated Human TIGIT Protein (TIG-HM410B)
Biotinylated Human TIGIT Protein (TIG-HM410B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human TIGIT, His-Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD155, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Met22-Pro141
Catalog #TIG-HM410B
Regular price $577.50
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Product Description

Recombinant Biotinylated Human TIGIT Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met22-Pro141.

Product Background

TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.TIGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein

TIGIT

Synonyms

TIGIT; VSIG9; VSTM3; WUCAM

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Met22-Pro141

Molecular Weight

The protein has a predicted MW of 16.1 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Human TIGIT, His-Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD155, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human TIGIT, His-Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD155, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA. Immobilized Biotinylated Human TIGIT, His-Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD155, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA.
Biotinylated Human TIGIT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human TIGIT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human TIGIT is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human TIGIT is greater than 95% as determined by SEC-HPLC.

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