Name: Biotinylated Human TIGIT Protein (TIG-HM410B)
Brand: KactusBio
SKU: TIG-HM410B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human TIGIT Protein (TIG-HM410B)

Immobilized Biotinylated Human TIGIT, His-Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD155, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA.

Biotinylated Human TIGIT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human TIGIT is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human TIGIT, His-Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD155, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Met22-Pro141

Catalog #TIG-HM410B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human TIGIT Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met22-Pro141.
Product Background	TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.TIGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	TIGIT
Synonyms	TIGIT; VSIG9; VSTM3; WUCAM
Accession	Q495A1
Species	Human
Biotinylated	yes
Amino Acid Range	Met22-Pro141
Molecular Weight	The protein has a predicted MW of 16.1 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human TIGIT, His-Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD155, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human TIGIT, His-Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD155, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA.
	Biotinylated Human TIGIT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human TIGIT is greater than 95% as determined by SEC-HPLC.