Biotinylated Human TLR3 Protein (TLR-HM403B)
|Recombinant Biotinylated Human TLR3 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ser23-Glu703.
|TLR3 is expressed in the central nervous system (CNS), where it is required to control HSV-1, which spreads from the epithelium to the CNS via cranial nerves. TLR3 is also expressed in epithelial and dendritic cells, which apparently use TLR3-independent pathways to prevent further dissemination of HSV-1 and to provide resistance to other pathogens in TLR3-deficient patients. Human TLR3 appears to be redundant in host defense to most microbes but is vital for natural immunity to HSV-1 in the CNS, which suggests that neurotropic viruses have contributed to the evolutionary maintenance of TLR3.
|The protein has a predicted MW of 80.25 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Tris-Bis PAGE result.
|Immobilized Biotinylated Human TLR3, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-TLR3 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA. See testing image for detail.
|> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
|Less than 1EU per ug by the LAL method.
|Shipped with dry ice.
|Supplied as 0.22 um filtered solution in PBS (pH 7.4).
|Stability And Storage
|Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.