Product Description |
Recombinant Biotinylated Human TLR3 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ser23-Glu703. |
Product Background |
TLR3 is expressed in the central nervous system (CNS), where it is required to control HSV-1, which spreads from the epithelium to the CNS via cranial nerves. TLR3 is also expressed in epithelial and dendritic cells, which apparently use TLR3-independent pathways to prevent further dissemination of HSV-1 and to provide resistance to other pathogens in TLR3-deficient patients. Human TLR3 appears to be redundant in host defense to most microbes but is vital for natural immunity to HSV-1 in the CNS, which suggests that neurotropic viruses have contributed to the evolutionary maintenance of TLR3. |
Product Category |
Recombinant Protein |
Target |
TLR3 |
Synonyms |
TLR3; CD283; IIAE2 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ser23-Glu703 |
Molecular Weight |
The protein has a predicted MW of 80.25 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human TLR3, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-TLR3 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Biotinylated Human TLR3, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-TLR3 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA. |
|
|
Biotinylated Human TLR3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Biotinylated Human TLR3 is greater than 95% as determined by SEC-HPLC. |
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