Biotinylated Mouse GARP&Latent TGF beta 1 Complex Protein (GAT-MM401B)
  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse ITGAV&ITGB6, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse GARP&Latent TGF beta 1 Complex, His Tag with the EC50 of 0.12ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala18-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1)
Catalog #GAT-MM401B
Regular price $675.00
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Product Description
Recombinant Biotinylated Mouse GARP&Latent TGF beta 1 Complex Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ala18-Leu628(GARP)&Leu30-Ser390(Latent TGF beta 1).
Product Background
GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.
Product Category
Recombinant Protein
Target
GARP&Latent TGF beta 1 Complex
Synonyms
LRRC32; GARP; LAP; TGF-beta-1; LRRC32&TGF-beta 1; LRRC32&TGFB1
Accession
Species
Mouse
Biotinylated
yes
Amino Acid Range
Ala18-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1)
Molecular Weight
The protein has a predicted MW of 66.6 kDa (GARP)&44.2 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 72-76 kDa (GARP)&13 kDa&45-47 kDa (Latent TGF beta 1) based on Bis-Tris PAGE result.
Activity
Immobilized Mouse ITGAV&ITGB6, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse GARP&Latent TGF beta 1 Complex, His Tag with the EC50 of 0.12ug/ml determined by ELISA. See testing image for detail.
Product Tag
N-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse ITGAV&ITGB6, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse GARP&Latent TGF beta 1 Complex, His Tag with the EC50 of 0.12ug/ml determined by ELISA.
Biotinylated Mouse GARP&Latent TGF beta 1 Complex on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Mouse GARP&Latent TGF beta 1 Complex is greater than 95% as determined by SEC-HPLC.

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