Name: Biotinylated Mouse GARP&Latent TGF beta 1 Complex Protein (GAT-MM401B)
Brand: KactusBio
SKU: GAT-MM401B-100UG
Price: 742.5 USD
Availability: InStock

Biotinylated Mouse GARP&Latent TGF beta 1 Complex Protein (GAT-MM401B)

Immobilized Mouse ITGAV&ITGB6, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse GARP&Latent TGF beta 1 Complex, His Tag with the EC50 of 0.12ug/ml determined by ELISA.

Biotinylated Mouse GARP&Latent TGF beta 1 Complex on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Mouse GARP&Latent TGF beta 1 Complex is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Mouse ITGAV&ITGB6, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse GARP&Latent TGF beta 1 Complex, His Tag with the EC50 of 0.12ug/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Ala18-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1)

Catalog #GAT-MM401B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Mouse GARP&Latent TGF beta 1 Complex Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ala18-Leu628(GARP)&Leu30-Ser390(Latent TGF beta 1).
Product Background	GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.
Product Category	Recombinant Protein / Antibody discovery

Protein	GARP&Latent TGF beta 1 Complex
Synonyms	LRRC32; GARP; LAP; TGF-beta-1; LRRC32&TGF-beta 1; LRRC32&TGFB1; TGF-β-1; LRRC32&TGF-β 1; GARP&Latent TGF Beta; GARP&Latent TGF Beta 1
Accession	G3XA59(GARP)&P04202(Latent TGF beta 1)
Species	Mouse
Biotinylated	yes
Amino Acid Range	Ala18-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1)
Molecular Weight	The protein has a predicted MW of 66.6 kDa (GARP)&44.2 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 72-76 kDa (GARP)&13 kDa&45-47 kDa (Latent TGF beta 1) based on Bis-Tris PAGE result.
Activity	Immobilized Mouse ITGAV&ITGB6, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse GARP&Latent TGF beta 1 Complex, His Tag with the EC50 of 0.12ug/ml determined by ELISA. See testing image for detail.
Product Tag	N-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Mouse ITGAV&ITGB6, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse GARP&Latent TGF beta 1 Complex, His Tag with the EC50 of 0.12ug/ml determined by ELISA.
	Biotinylated Mouse GARP&Latent TGF beta 1 Complex on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Mouse GARP&Latent TGF beta 1 Complex is greater than 95% as determined by SEC-HPLC.