Biotinylated Mouse MSLN/Mesothelin Protein (Primary Amine Labeling) (MSL-MM180B)
|Recombinant Biotinylated Mouse MSLN/Mesothelin Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Asp298-Ser600.
|Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.
|MSLN; CAK1; Mesothelin; MPF; MPFSMRP; SMR
|The protein has a predicted MW of 35.2 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.
|Immobilized Human CA125, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse MSLN, His Tag with the EC50 of 0.58ug/ml determined by ELISA. See testing image for detail.
|> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
|Less than 1EU per ug by the LAL method.
|Shipped at ambient temperature.
|Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
|Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
|Stability And Storage
|Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.