Biotinylated Mouse MSLN/Mesothelin Protein (Primary Amine Labeling) (MSL-MM180B)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human CA125, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse MSLN, His Tag with the EC50 of 0.58ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Asp298-Ser600

Catalog: MSL-MM180B

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Product Description Recombinant Biotinylated Mouse MSLN/Mesothelin Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Asp298-Ser600.

Product Background

 Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.

Product Category

 Recombinant Protein

Target

 MSLN/Mesothelin
Synonyms MSLN; CAK1; Mesothelin; MPF; MPFSMRP; SMR

Gene/Protein ID

 Q61468-1

Species

 Mouse

Exact Sequence

 Asp298-Ser600
Molecular Weight The protein has a predicted MW of 35.2 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Human CA125, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse MSLN, His Tag with the EC50 of 0.58ug/ml determined by ELISA. See testing image for detail.

Product Tag

 C-His

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CA125, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse MSLN, His Tag with the EC50 of 0.58ug/ml determined by ELISA.
The purity of Biotinylated Mouse MSLN is greater than 90% as determined by SEC-HPLC.
Biotinylated Mouse MSLN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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