FITC-equivalent Human CD20 Protein-Nanodisc (CD2-HM1N37)
  • Express System: HEK293
  • Product Tag: C-His
  • Activity: Immobilized Rituximab, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for FITC-equivalent Human CD20 Nanodisc, His Tag with the EC50 of 14.6ng/ml determined by ELISA (QC Test). The affinity constant of 3.09 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Met1-Pro297
Catalog #CD2-HM1N37
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Product Description
 Recombinant FITC-equivalent Human CD20 Protein-Nanodisc is expressed from HEK293 with His tag at the C-terminus.It contains Met1-Pro297.
Product Background
 B-lymphocyte antigen CD20 or CD20 is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R , CD117) and progressively increasing in concentration until maturity.CD20 is the target of the monoclonal antibodies rituximab, ocrelizumab, obinutuzumab, ofatumumab, ibritumomab tiuxetan, tositumomab, and ublituximab, which are all active agents in the treatment of all B cell lymphomas, leukemias, and B cell-mediated autoimmune diseases.
Product Category
 Recombinant Protein
Target
 CD20 Nanodisc
Synonyms
 MS4A1; CD20; MS4A-1; B1; Bp35; CD20 receptor; CD20S7; CVID5; LEU-16; Ly-44; MS4A2; S7
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Met1-Pro297
Molecular Weight
 The protein has a predicted MW of 34.3 kDa kDa.
Activity
 Immobilized Rituximab, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for FITC-equivalent Human CD20 Nanodisc, His Tag with the EC50 of 14.6ng/ml determined by ELISA (QC Test). The affinity constant of 3.09 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Fluorophore
 FITC
Express System
 HEK293
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS (pH 7.4). Notice: Not recommended for immunization.
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
FITC-equivalent Human CD20 Nanodisc, His Tag captured on CM5 Chip via anti-his antibody can bind Rituximab, hFc Tag with an affinity constant of 3.09 nM as determined in SPR assay (Biacore T200).
FITC-equivalent Human CD20 Nanodisc, His Tag captured on CM5 Chip via anti-his antibody can bind Ofatumumab, hFc Tag with an affinity constant of 153.07 nM as determined in SPR assay (Biacore T200).
Immobilized Rituximab, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for FITC-equivalent Human CD20 Nanodisc, His Tag with the EC50 of 14.6ng/ml determined by ELISA (QC Test).
Immobilized FITC-equivalent Human CD20 Nanodisc, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Obinutuzumab, hFc Tag with the EC50 of 35.3ng/ml determined by ELISA.

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