Product Description |
Recombinant FITC-equivalent Human CD20 Protein-Nanodisc is expressed from HEK293 with His tag at the C-terminus.It contains Met1-Pro297. |
Product Background |
B-lymphocyte antigen CD20 or CD20 is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R , CD117) and progressively increasing in concentration until maturity.CD20 is the target of the monoclonal antibodies rituximab, ocrelizumab, obinutuzumab, ofatumumab, ibritumomab tiuxetan, tositumomab, and ublituximab, which are all active agents in the treatment of all B cell lymphomas, leukemias, and B cell-mediated autoimmune diseases. |
Product Category |
Recombinant Protein |
Target |
CD20 Nanodisc |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met1-Pro297 |
Molecular Weight |
The protein has a predicted MW of 34.3 kDa kDa. |
Activity |
Immobilized Rituximab, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for FITC-equivalent Human CD20 Nanodisc, His Tag with the EC50 of 14.6ng/ml determined by ELISA (QC Test). The affinity constant of 3.09 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Fluorophore |
FITC |
Express System |
HEK293 |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in PBS (pH 7.4). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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FITC-equivalent Human CD20 Nanodisc, His Tag captured on CM5 Chip via anti-his antibody can bind Rituximab, hFc Tag with an affinity constant of 3.09 nM as determined in SPR assay (Biacore T200). |
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FITC-equivalent Human CD20 Nanodisc, His Tag captured on CM5 Chip via anti-his antibody can bind Ofatumumab, hFc Tag with an affinity constant of 153.07 nM as determined in SPR assay (Biacore T200). |
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Immobilized Rituximab, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for FITC-equivalent Human CD20 Nanodisc, His Tag with the EC50 of 14.6ng/ml determined by ELISA (QC Test). |
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Immobilized FITC-equivalent Human CD20 Nanodisc, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Obinutuzumab, hFc Tag with the EC50 of 35.3ng/ml determined by ELISA. |
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