Biotinylated Human CD24 Protein (Primary Amine Labeling) (CD2-HM324B)
Biotinylated Human CD24 Protein (Primary Amine Labeling) (CD2-HM324B)
Biotinylated Human CD24 Protein (Primary Amine Labeling) (CD2-HM324B)
Biotinylated Human CD24 Protein (Primary Amine Labeling) (CD2-HM324B)
Biotinylated Human CD24 Protein (Primary Amine Labeling) (CD2-HM324B)
Biotinylated Human CD24 Protein (Primary Amine Labeling) (CD2-HM324B)
  • Express System: HEK293
  • Product Tag: C-mFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Anti-CD24 Antibody at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD24, mFc Tag with the EC50 of 0.31ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ser27-Gly59
Catalog #CD2-HM324B
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Product Description
 Recombinant Biotinylated Human CD24 Protein (Primary Amine Labeling) is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Ser27-Gly59.
Product Background
 CD24 is a sialoglycoprotein expressed at the surface of most B lymphocytes and differentiating neuroblasts. It is also expressed on neutrophils and neutrophil precursors from the myelocyte stage onwards. The potential for targeting CD24 in cancer therapy seems promising, as CD24 is overexpressed in many human cancers.
Product Category
 Recombinant Protein
Target
 CD24
Synonyms
 CD 24; CD24 molecule; CD24A; FLJ22950; FLJ43543; MGC75043
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Ser27-Gly59
Molecular Weight
 The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Anti-CD24 Antibody at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD24, mFc Tag with the EC50 of 0.31ug/ml determined by ELISA. See testing image for detail.
Product Tag
 C-mFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-CD24 Antibody at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD24, mFc Tag with the EC50 of 0.31ug/ml determined by ELISA.
Biotinylated Human CD24 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Biotinylated Human CD24 is greater than 95% as determined by SEC-HPLC.

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