SEC-HPLC chromatogram of biotinylated human CD24 protein showing a sharp main peak at retention time 6.498 minutes, indicating high purity with minimal aggregation.
Biotinylated Human CD24 Protein (Primary Amine Labeling) (CD2-HM324B)
Biotinylated Human CD24 Protein (Primary Amine Labeling) (CD2-HM324B)
  • Express System: HEK293
  • Product Tag: C-mFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Anti-CD24 Antibody at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD24, mFc Tag with the EC50 of 0.31ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ser27-Gly59
Catalog #CD2-HM324B
Regular price $577.50
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Biotinylated Human CD24 Protein (Primary Amine Labeling) (CD2-HM324B) Summary

Product Description

Recombinant Biotinylated Human CD24 Protein (Primary Amine Labeling) is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Ser27-Gly59.

Product Background

CD24 is a sialoglycoprotein expressed at the surface of most B lymphocytes and differentiating neuroblasts. It is also expressed on neutrophils and neutrophil precursors from the myelocyte stage onwards. The potential for targeting CD24 in cancer therapy seems promising, as CD24 is overexpressed in many human cancers.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Biotinylated Human CD24 Protein (Primary Amine Labeling) (CD2-HM324B) Specifications/Details

Protein
CD24
Synonyms
CD 24; CD24 molecule; CD24A; FLJ22950; FLJ43543; MGC75043
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Ser27-Gly59
Molecular Weight
The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Anti-CD24 Antibody at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD24, mFc Tag with the EC50 of 0.31ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-mFc
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Anti-CD24 Antibody at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD24, mFc Tag with the EC50 of 0.31ug/ml determined by ELISA. Immobilized Anti-CD24 Antibody at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD24, mFc Tag with the EC50 of 0.31ug/ml determined by ELISA.
Biotinylated Human CD24 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Biotinylated Human CD24 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Biotinylated Human CD24 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human CD24 is greater than 95% as determined by SEC-HPLC.
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