Product Description |
Recombinant Biotinylated Human CD24 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser27-Gly59. |
Product Background |
CD24 is a sialoglycoprotein expressed at the surface of most B lymphocytes and differentiating neuroblasts. It is also expressed on neutrophils and neutrophil precursors from the myelocyte stage onwards. The potential for targeting CD24 in cancer therapy seems promising, as CD24 is overexpressed in many human cancers. |
Product Category |
Recombinant Protein |
Target |
CD24 |
Synonyms |
CD 24; CD24 molecule; CD24A; FLJ22950; FLJ43543; MGC75043 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ser27-Gly59 |
Molecular Weight |
The protein has a predicted MW of 6.5 kDa. Due to glycosylation, the protein migrates to 25-40 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human CD24, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD24 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Biotinylated Human CD24, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD24 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). |
|
|
Biotinylated Human CD24 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Biotinylated Human CD24 is greater than 95% as determined by SEC-HPLC. |
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