Biotinylated Human CD24 Protein (CD2-HM424B)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Biotinylated: yes
Activity: Immobilized Biotinylated Human CD24, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD24 Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ser27-Gly59

Catalog: CD2-HM424B

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Product Description	Recombinant Biotinylated Human CD24 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser27-Gly59.
Product Background	CD24 is a sialoglycoprotein expressed at the surface of most B lymphocytes and differentiating neuroblasts. It is also expressed on neutrophils and neutrophil precursors from the myelocyte stage onwards. The potential for targeting CD24 in cancer therapy seems promising, as CD24 is overexpressed in many human cancers.
Product Category	Recombinant Protein

Target	CD24
Gene/Protein ID	P25063-1
Species	Human
Exact Sequence	Ser27-Gly59
Molecular Weight	The protein has a predicted MW of 6.5 kDa. Due to glycosylation, the protein migrates to 25-40 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Biotinylated Human CD24, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD24 Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Biotinylated Human CD24, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD24 Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA.
	The purity of Biotinylated Human CD24 is greater than 95% as determined by SEC-HPLC.
	Biotinylated Human CD24 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.