Biotinylated Human CD24 Protein (CD2-HM424B)
Biotinylated Human CD24 Protein (CD2-HM424B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human CD24, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD24 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ser27-Gly59
Catalog #CD2-HM424B
Regular price $577.50
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Product Description

Recombinant Biotinylated Human CD24 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser27-Gly59.

Product Background

CD24 is a sialoglycoprotein expressed at the surface of most B lymphocytes and differentiating neuroblasts. It is also expressed on neutrophils and neutrophil precursors from the myelocyte stage onwards. The potential for targeting CD24 in cancer therapy seems promising, as CD24 is overexpressed in many human cancers.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others
Protein
CD24
Synonyms
CD 24; CD24 molecule; CD24A; FLJ22950; FLJ43543; MGC75043
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Ser27-Gly59
Molecular Weight
The protein has a predicted MW of 6.5 kDa. Due to glycosylation, the protein migrates to 25-40 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human CD24, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD24 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human CD24, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD24 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human CD24, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD24 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test).
Biotinylated Human CD24 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human CD24 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CD24 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human CD24 is greater than 95% as determined by SEC-HPLC.
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