Cynomolgus B7-H2/ICOSLG Protein (BH7-CM172)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human ICOS, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus B7-H2, His Tag with the EC50 of 12.6ng/ml determined by ELISA (QC Test). The affinity constant of 5.42 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Asp19-Thr256
Catalog #BH7-CM172
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Product Description
 Recombinant Cynomolgus B7-H2/ICOSLG Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp19-Thr256.
Product Background
 B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells.
Product Category
 Recombinant Protein
Target
 B7-H2/ICOSLG
Synonyms
 CD275; B7H2; B7-H2; B7RP-1; GL50; ICOSL; ICOS-L; ICOSLG; B7RP1; LICOS; ICOS ligand; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Asp19-Thr256
Molecular Weight
 The protein has a predicted MW of 27.7 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human ICOS, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus B7-H2, His Tag with the EC50 of 12.6ng/ml determined by ELISA (QC Test). The affinity constant of 5.42 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus B7-H2, His Tag with an affinity constant of 5.42 nM as determined in SPR assay (Biacore T200).
Immobilized Human ICOS, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus B7-H2, His Tag with the EC50 of 12.6ng/ml determined by ELISA (QC Test).
Cynomolgus B7-H2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus B7-H2 is greater than 95% as determined by SEC-HPLC.

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