Cynomolgus CD27 Ligand/CD70 Protein (CD7-CM170)

  • Express System: Expi293
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus CD27 Ligand, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque CD27, hFc Tag with the EC50 of 41.5ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln39-Pro194

Catalog: CD7-CM170

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Product Description Recombinant Cynomolgus CD27 Ligand/CD70 Protein is expressed from Expi293 with His tag at the N-terminal.; It contains Gln39-Pro194.
Product Category Recombinant Protein
Target CD27 Ligand/CD70
Synonyms CD70 molecule; CD70; TNFSF7; CD27 Ligand; CD27-L; CD27LG;  Ki-24 antigen; TNFSF7G
Gene/Protein ID G7PYU6-1
Species Cynomolgus
Exact Sequence Gln39-Pro194
Molecular Weight The protein has a predicted MW of 18.4 kDa. Due to glycosylation, the protein migrates to 60-90 kDa based on Tris-Bis PAGE result.
Activity Immobilized Cynomolgus CD27 Ligand, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque CD27, hFc Tag with the EC50 of 41.5ng/ml determined by ELISA. See testing image for detail.
Product Tag N-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus CD27 Ligand, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque CD27, hFc Tag with the EC50 of 41.5ng/ml determined by ELISA.
Cynomolgus CD27 Ligand/CD70 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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