Product Description |
Recombinant Cynomolgus CD27 Ligand/CD70 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Gln39-Pro194. |
Product Background |
CD70, also named CD27 ligand (CD27L), is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7.- CD70 is a cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets |
Protein |
CD27 Ligand/CD70 |
Synonyms |
CD70 molecule; CD70; TNFSF7; CD27 Ligand; CD27-L; CD27LG;-Ki-24 antigen; TNFSF7G |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Gln39-Pro194 |
Molecular Weight |
The protein has a predicted MW of 18.4 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus CD27 Ligand, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque CD27, hFc Tag with the EC50 of 100.2ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
N-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE; |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Cynomolgus CD27 Ligand, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque CD27, hFc Tag with the EC50 of 100.2ng/ml determined by ELISA (QC Test). |
|
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Cynomolgus CD27 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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