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Cynomolgus CD34 Protein (CD3-CM134) Summary
Product Description |
Recombinant Cynomolgus CD34 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asn39-Thr295. |
Product Background |
CD34 is a transmembrane phosphoglycoprotein, first identified on hematopoietic stem and progenitor cells. Clinically, it is associated with the selection and enrichment of hematopoietic stem cells for bone marrow transplants. Due to these historical and clinical associations, CD34 expression is almost ubiquitously related to hematopoietic cells, and it is a common misconception that CD34-positive (CD34()) cells in nonhematopoietic samples represent hematopoietic contamination. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Cynomolgus CD34 Protein (CD3-CM134) Specifications/Details
Protein |
CD34 |
Synonyms |
CD34; RP11-328D5.2; CD34 molecule; HPCA1 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Asn39-Thr295 |
Molecular Weight |
The protein has a predicted MW of 28.37 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Cynomolgus CD34 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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