Cynomolgus GM-CSF R alpha Protein (GSF-CM1RA)

SKU: GSF-CM1RA-100UG

Cynomolgus GM-CSF R alpha Protein (GSF-CM1RA)

SKU: GSF-CM1RA-100UG
ELISA binding curve showing the interaction between Cynomolgus GM-CSF R alpha (His tag) and anti-GM-CSF R alpha antibody. The signal increases with antibody concentration and plateaus, indicating specific binding.
Cynomolgus GM-CSF R alpha Protein (GSF-CM1RA)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus GM-CSF R alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GM-CSF R alpha Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA.
  • Amino Acid Range: Leu20-Ser317
Catalog #GSF-CM1RA
Regular price $346.50
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Cynomolgus GM-CSF R alpha Protein (GSF-CM1RA) Summary

Product Description

Recombinant Cynomolgus GM-CSF R alpha Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu20-Ser317.

Product Background

The receptor for granulocyte-macrophage colony-stimulating factor (GM-CSF) is composed of an alpha and beta subunit, which together form the high-affinity receptor. The alpha subunit by itself binds ligand at low affinity, whereas the isolated beta subunit does not bind GM-CSF. It is generally believed that the high-affinity receptor is responsible for the multiple functions of GM-CSF and that the isolated alpha subunit (GMR alpha) does not transduce a signal.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Cynomolgus GM-CSF R alpha Protein (GSF-CM1RA) Specifications/Details

Protein
GM-CSF R alpha
Synonyms
GM-CSF-R-alpha; GMCSFR-alpha; GMR-alpha; CSF2R; CSF2RY; CSF2RA; CDw116; CD116; CSF2RAX; CSF2RAY; CSF2RX; GMCSFR; GMR; SMDP4
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Leu20-Ser317
Molecular Weight
The protein has a predicted MW of 35.47 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus GM-CSF R alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GM-CSF R alpha Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA.
Product Tag
C-His

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Cynomolgus GM-CSF R alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GM-CSF R alpha Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA. Immobilized Cynomolgus GM-CSF R alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GM-CSF R alpha Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA.
Cynomolgus GM-CSF R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus GM-CSF R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus GM-CSF R alpha is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus GM-CSF R alpha is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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