Cynomolgus GM-CSF R alpha Protein (GSF-CM1RA)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus GM-CSF R alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GM-CSF R alpha Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu20-Ser317

Catalog: GSF-CM1RA

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Product Description Recombinant Cynomolgus GM-CSF R alpha Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu20-Ser317.

Product Background

In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua.

Product Category

Recombinant Protein

Target

GM-CSF R alpha

Gene/Protein ID

A0A2K5UC70

Species

Cynomolgus

Exact Sequence

Leu20-Ser317
Molecular Weight The protein has a predicted MW of 35.47 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Cynomolgus GM-CSF R alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GM-CSF R alpha Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus GM-CSF R alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GM-CSF R alpha Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA.
The purity of Cynomolgus GM-CSF R alpha is greater than 95% as determined by SEC-HPLC.
Cynomolgus GM-CSF R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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