Product Description |
Recombinant Cynomolgus HPX Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asn24-Tyr462. |
Product Background |
Hemopexin (HPX) serves as scavenger and transporter of toxic plasma heme to the liver. HPX is formed by two four-bladed beta-propeller domains, resembling two thick disks that lock together at a 90 degrees angle. The heme is bound between the two beta-propeller domains in a pocket formed by the interdomain linker peptide.HPX, acting not only as a heme carrier but also displaying transient heme-based ligand binding and (pseudo-)enzymatic properties, could be considered a 'chronosteric' heme-protein. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
HPX |
Synonyms |
Hemopexin;Hpx;Hpxn |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Asn24-Tyr462 |
Molecular Weight |
The protein has a predicted MW of 50.30 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM MES, 150mM NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM MES, 150mM NaCl (pH 6.0). |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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