Cynomolgus HPX Protein (HPX-CM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Exact Sequence: Asn24-Tyr462

Catalog: HPX-CM101

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Product Description Recombinant Cynomolgus HPX Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asn24-Tyr462.

Product Background

 Hemopexin (HPX) serves as scavenger and transporter of toxic plasma heme to the liver. HPX is formed by two four-bladed beta-propeller domains, resembling two thick disks that lock together at a 90 degrees angle. The heme is bound between the two beta-propeller domains in a pocket formed by the interdomain linker peptide.HPX, acting not only as a heme carrier but also displaying transient heme-based ligand binding and (pseudo-)enzymatic properties, could be considered a 'chronosteric' heme-protein.

Product Category

 Recombinant Protein

Target

 HPX

Gene/Protein ID

 A0A2K5WVL1

Species

 Cynomolgus

Exact Sequence

 Asn24-Tyr462
Molecular Weight The protein has a predicted MW of 50.30 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Tris-Bis PAGE result.

Product Tag

 C-His

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 20mM MES, 150mM NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM MES, 150mM NaCl (pH 6.0).
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
The purity of Cynomolgus HPX is greater than 95% as determined by SEC-HPLC.
Cynomolgus HPX on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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