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Cynomolgus IL-2 R gamma/CD132 Protein (CD1-CM132) Summary
Product Description |
Recombinant Cynomolgus IL-2 R gamma/CD132 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu23-Asn254. |
Product Background |
The gamma chain of the high affinity functional human IL-2 receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 232 residue extracellular domain, a 29 residue transmembrane domain and an 86 residue cytoplasmic domain. Although IL-2 R gamma by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Cynomolgus IL-2 R gamma/CD132 Protein (CD1-CM132) Specifications/Details
Protein |
IL-2 R gamma/CD132 |
Synonyms |
CD132; CIDX; IL-2 R gamma; IL2RG; IMD4; P64; SCIDX; SCIDX1; gammaC |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Leu23-Asn254 |
Molecular Weight |
The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 50-68 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Cynomolgus IL-2 R gamma on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
|
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The purity of Cynomolgus IL-2 R gamma is greater than 90% as determined by SEC-HPLC. |
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