Cynomolgus Kallikrein 3/PSA-Protein (active form), Ultra Low Endotoxin (KLK-CM13A-UL)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Measured by its ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p-Nitroanilide (Suc-RPY-pNA). The specific activity is >100 pmol/min/ug.
  • Amino Acid Range: Ile25-Pro261
Catalog #KLK-CM13A-UL
Regular price $375.00
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Cynomolgus Kallikrein 3/PSA-Protein (active form), Ultra Low Endotoxin (KLK-CM13A-UL) Summary

Product Description

Recombinant Cynomolgus Kallikrein 3/PSA-Protein (active form) is expressed from HEK293 with His tag at the C-terminus.It contains Ile25-Pro261.

Product Background

Kallikrein-related peptidase 3 (KLK3), also known as prostate-specific antigen (PSA), is the most useful biomarker for prostate cancer (PCa). KLK3 is suggested to play a role in regulating cancer growth through anti-angiogenic activity in vivo and in vitro.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery/Others

Cynomolgus Kallikrein 3/PSA-Protein (active form), Ultra Low Endotoxin (KLK-CM13A-UL) Specifications/Details

Protein
Kallikrein 3/PSA
Synonyms
PSA; Kallikrein 3; Kallikrein-3; P-30 antigen; Semenogelase; KLK3; APS
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Ile25-Pro261
Molecular Weight
The protein has a predicted MW of 27.76 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Bis-Tris PAGE result.
Activity
Measured by its ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p-Nitroanilide (Suc-RPY-pNA). The specific activity is >100 pmol/min/ug.
Product Tag
C-His

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in 50mM Tris, 150mM NaCl, 8% trehalose, 0.05%Brij-35 (pH 7.5).
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Cynomolgus Kallikrein 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus Kallikrein 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus Kallikrein 3 is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus Kallikrein 3 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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