Cynomolgus MICA Protein (MIC-CM10A)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus MICA, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.14ug/ml determined by ELISA. See testing image for detail.
Exact Sequence: Glu1-Trp284

Catalog: MIC-CM10A

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Product Description	Recombinant Cynomolgus MICA Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu1-Trp284.
Product Background	MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig‑like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin..
Product Category	Recombinant Protein

Target	MICA
Gene/Protein ID	AAO24115.1
Species	Cynomolgus
Exact Sequence	Glu1-Trp284
Molecular Weight	The protein has a predicted MW of 33.53 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus MICA, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.14ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Cynomolgus MICA, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.14ug/ml determined by ELISA.
	The purity of Cynomolgus MICA is greater than 95% as determined by SEC-HPLC.
	Cynomolgus MICA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.