Cynomolgus MICA Protein (MIC-CM10A)
Cynomolgus MICA Protein (MIC-CM10A)
Cynomolgus MICA Protein (MIC-CM10A)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus MICA, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 64.5ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Glu1-Trp284
Catalog #MIC-CM10A
Regular price $315.00
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Product Description
Recombinant Cynomolgus MICA Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu1-Trp284.
Product Background
MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig‑like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin..
Product Category
Recombinant Protein
Target
MICA
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Glu1-Trp284
Molecular Weight
The protein has a predicted MW of 33.53 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus MICA, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 64.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus MICA, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 64.5ng/ml determined by ELISA (QC Test).
Cynomolgus MICA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus MICA is greater than 95% as determined by SEC-HPLC.

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