• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus PTK7, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-PTK7 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA.
  • Amino Acid Range: Ala39-Thr712
Catalog #CCK-CM104-UL
Price
Regular price $381.00
Regular price Sale price $381.00
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Size
Avantor VWR Fisher Scientific

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Cynomolgus PTK7/CCK4 Protein, Ultra Low Endotoxin (CCK-CM104-UL) Summary

Product Description

 Recombinant Cynomolgus PTK7/CCK4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala39-Thr712.

Product Background

 Protein Tyrosine Kinase 7 (PTK7) is as a critical regulator of canonical and non-canonical Wnt-signaling during embryonic development and cancer cell formation. Disrupting PTK7 activity perturbs vertebrate nervous system development, and also promotes human cancer formation. Observations in different model systems suggest a complex cross-talk between PTK7 protein and Wnt signaling.

Product Category

 Recombinant Protein / Antibody discovery / ADC targets

Cynomolgus PTK7/CCK4 Protein, Ultra Low Endotoxin (CCK-CM104-UL) Specifications/Details

Protein
 PTK7/CCK4
Synonyms
 CCK-4; PTK7;CCK4
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Ala39-Thr712
Molecular Weight
 The protein has a predicted MW of 75.73 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus PTK7, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-PTK7 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA.
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Cynomolgus PTK7, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-PTK7 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. Immobilized Cynomolgus PTK7, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-PTK7 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA.
Cynomolgus PTK7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus PTK7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus PTK7 is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus PTK7 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...