Cynomolgus/Rhesus macaque EpCAM/TROP1 Protein (CAM-CM0EP)
  • Express System: HEK293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Rhesus macaque/Cynomolgus EpCAM, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-EpCAM Antibody, hFc Tag with the EC50 of 0.14ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln24-Lys265
Catalog #CAM-CM0EP
Regular price $300.00
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Product Description
Recombinant Cynomolgus/Rhesus macaque EpCAM/TROP1 Protein is expressed from HEK293 without tag.It contains Gln24-Lys265.
Product Background
Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.
Product Category
Recombinant Protein
Target
EpCAM/TROP1
Accession
Species
Cynomolgus/Rhesus macaque
Biotinylated
no
Amino Acid Range
Gln24-Lys265
Molecular Weight
The protein has a predicted MW of 27.38 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Rhesus macaque/Cynomolgus EpCAM, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-EpCAM Antibody, hFc Tag with the EC50 of 0.14ug/ml determined by ELISA. See testing image for detail.
Product Tag
No Tag
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Rhesus macaque/Cynomolgus EpCAM, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-EpCAM Antibody, hFc Tag with the EC50 of 0.14ug/ml determined by ELISA.
Cynomolgus/Rhesus macaque EpCAM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus/Rhesus macaque EpCAM is greater than 95% as determined by SEC-HPLC.

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