- Express System: HEK293
- Product Tag: No Tag
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Rhesus macaque/Cynomolgus EpCAM, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EpCAM Antibody, hFc Tag with the EC50 of 0.23ug/ml determined by ELISA (QC Test). See testing image for detail.
- Amino Acid Range: Gln24-Lys265
Product Description |
Recombinant Cynomolgus/Rhesus macaque EpCAM/TROP1 Protein is expressed from HEK293 without tag.It contains Gln24-Lys265. |
Product Background |
Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain. |
Product Category |
Recombinant Protein |
Target |
EpCAM/TROP1 |
Synonyms |
CD326; EGP; EGP-2; EGP314; EGP40; EpCAM; ACSTD1; DIAR5; gp40; HNPCC8; MOC31; TACST-1; TACSTD1; TROP1; ESA; KSA; MIC18; MK1 |
Accession |
|
Species |
Cynomolgus/Rhesus macaque |
Biotinylated |
no |
Amino Acid Range |
Gln24-Lys265 |
Molecular Weight |
The protein has a predicted MW of 27.38 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Rhesus macaque/Cynomolgus EpCAM, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EpCAM Antibody, hFc Tag with the EC50 of 0.23ug/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
No Tag |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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