Human Adenylosuccinate Lyase-Protein (ASL-HE001) Summary
Product Description |
Recombinant Human Adenylosuccinate Lyase-Protein is expressed from E.coli with His tag at the N-Terminus.It contains Ala2-Leu484. |
Product Background |
Adenylosuccinate lyase (ADSL) is an essential enzyme for de novo purine biosynthesis, ADSL functions in de novo purine synthesis (DNPS) and the purine nucleotide cycle. Adenylosuccinate lyase ADSL) deficiency is a defect of purine metabolism affecting purinosome assembly and reducing metabolite fluxes through purine de novo synthesis and purine nucleotide recycling pathways. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human Adenylosuccinate Lyase-Protein (ASL-HE001) Specifications/Details
Protein |
Adenylosuccinate Lyase |
Synonyms |
ADSL; ASase; Adenylosuccinase; AMPS; ASL |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala2-Leu484 |
Molecular Weight |
The protein has a predicted MW of 56.28 kDa same as Bis-Tris PAGE result. |
Product Tag |
N-His |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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