Human Beta-NGF Protein (NGF-HM00B)

SKU: NGF-HM00B-100UG

Human Beta-NGF Protein (NGF-HM00B)

SKU: NGF-HM00B-100UG
Recombinant human Beta-NGF activity assay graph showing dose-dependent response. The sigmoidal curve plots Beta-NGF concentration (ng/ml) vs. RFU (Relative Fluorescence Units), indicating increased neurotrophic factor activity at higher doses.
Human Beta-NGF Protein (NGF-HM00B)
Human Beta-NGF Protein (NGF-HM00B)
Recombinant human Beta-NGF activity assay graph showing dose-dependent response. The sigmoidal curve plots Beta-NGF concentration (ng/ml) vs. RFU (Relative Fluorescence Units), indicating increased neurotrophic factor activity at higher doses.
Human Beta-NGF Protein (NGF-HM00B)
Human Beta-NGF Protein (NGF-HM00B)
  • Express System: HEK293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Amino Acid Range: Ser122-Ala241
Catalog #NGF-HM00B
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Size
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Human Beta-NGF Protein (NGF-HM00B) Summary

Product Description

 Recombinant Human Beta-NGF Protein is expressed from HEK293 without tag.It contains Ser122-Ala241.

Product Background

 Nerve growth factor-beta (β-NGF) is a polypeptide growth factor that regulates survival, growth, and differentiation of specific peripheral and central neurons via its high-affinity receptor, tyrosine kinase receptor A (TrKA), and low-affinity receptor, p75 neurotrophin receptor (p75NTR).

Product Category

 Recombinant Protein / Cell therapy / Cell culture / Cytokines

Human Beta-NGF Protein (NGF-HM00B) Specifications/Details

Protein
 Beta-NGF
Synonyms
 NGF; NGFB; Beta NGF; HSAN5; β-NGF
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ser122-Ala241
Molecular Weight
 The protein has a predicted MW of 13.49 kDa. Due to glycosylation, the protein migrates to 14-18 kDa based on Bis-Tris PAGE result.
Product Tag
 No Tag

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 20mM NaAc, 150mM Nacl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in 20mM NaAc (pH 5.5). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human Beta-NGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Beta-NGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2 - 2 ng/ml. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2 - 2 ng/ml.
Other Available Proteins:
Finding available proteins...
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