Product Description |
Recombinant Human Beta-NGF Protein is expressed from HEK293 without tag.It contains Ser122-Ala241. |
Product Background |
Nerve growth factor-beta (β-NGF) is a polypeptide growth factor that regulates survival, growth, and differentiation of specific peripheral and central neurons via its high-affinity receptor, tyrosine kinase receptor A (TrKA), and low-affinity receptor, p75 neurotrophin receptor (p75NTR). |
Product Category |
Recombinant Protein / Cell therapy / Cell culture / Cytokines |
Protein |
Beta-NGF |
Synonyms |
NGF; NGFB; Beta NGF; HSAN5; |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser122-Ala241 |
Molecular Weight |
The protein has a predicted MW of 13.49 kDa. Due to glycosylation, the protein migrates to 14-18 kDa based on Bis-Tris PAGE result. |
Product Tag |
No Tag |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 20mM NaAc, 150mM Nacl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in 20mM NaAc, 150mM Nacl (pH 5.5). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Human Beta-NGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2 - 2 ng/ml. |
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