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Human CA4/Carbonic Anhydrase IV Protein (CA4-HM204) Summary
Product Description |
Recombinant Human CA4/Carbonic Anhydrase IV Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Ala19-Ser284. |
Product Background |
Carbonic anhydrase IV is one of 12 active human isozymes and one of four expressed on the extracellular surfaces of certain endothelial and epithelial cells. It is unique in being attached to the plasma membrane by a glycosyl-phosphatiydyl-inositol (GPI) anchor rather than by a membrane-spanning domain. It is also uniquely resistant to high concentrations of sodium dodecyl sulfate (SDS), which allows purification from tissues by inhibitor affinity chromatography without contamination by other isozymes. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human CA4/Carbonic Anhydrase IV Protein (CA4-HM204) Specifications/Details
Protein |
CA4/Carbonic Anhydrase IV |
Synonyms |
CA-IV; CA IV; CA4 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala19-Ser284 |
Molecular Weight |
The protein has a predicted MW of 56.26 kDa. Due to glycosylation, the protein migrates to 57-67 kDa based on Bis-Tris PAGE result. |
Activity |
Measured by its esterase activity. The specific activity is > 5 pmol/min/µg. |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 25mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human CA4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human CA4 is greater than 95% as determined by SEC-HPLC. |
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