Human CD21 Protein (CD2-HM121)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD21, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD21 Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ile21-Arg971

Catalog: CD2-HM121

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Product Description	Recombinant Human CD21 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile21-Arg971.
Product Background	CD21 mRNA expression, an EBV-entry receptor, was transiently detected in NK cells after exosome treatment, and levels decreased after further culture. CD21 protein expression was also transiently transferred to NK cells after co-culture with an EBV-positive Burkitt lymphoma cell line (Raji) via trogocytosis. However, EBV did not infect NK cells through CD21-mediated trogocytosis.
Product Category	Recombinant Protein

Target	CD21
Synonyms	EBV receptor; CD21; C3DR; CR2; C3DRSLEB9; CVID7; SLEB9
Gene/Protein ID	P20023-1
Species	Human
Exact Sequence	Ile21-Arg971
Molecular Weight	The protein has a predicted MW of 105.3 kDa. Due to glycosylation, the protein migrates to 110-130 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD21, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD21 Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human CD21, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD21 Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA.
	The purity of Human CD21 is greater than 95% as determined by SEC-HPLC.
	Human CD21 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.