Human CD47 Protein (CD7-HM147)
Catalog: CD7-HM147
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Product Description | Recombinant Human CD47 is expressed from Expi293 with His tag at the C-terminal.It contains Gln19-Pro139. |
Product Category | Recombinant Protein |
Target | CD47 |
Synonyms | CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6 |
Gene/Protein ID | Q08722-1 |
Species | Human |
Exact Sequence | Gln19-Pro139 |
Molecular Weight | The protein has a predicted MW of 14.5 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human CD47, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 46.8ng/ml determined by ELISA. The affinity constant of 18.9nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag | C-His |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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