Human CD47 Protein (CD7-HM147)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human CD47, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 28.9ng/ml determined by ELISA. The affinity constant of 18.9nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gln19-Pro139

Catalog: CD7-HM147

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Product Description Recombinant Human CD47 is expressed from Expi293 with His tag at the C-terminal.It contains Gln19-Pro139.
Product Category Recombinant Protein
Target CD47
Synonyms CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6
Gene/Protein ID Q08722-1
Species Human
Exact Sequence Gln19-Pro139
Molecular Weight The protein has a predicted MW of 14.5 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human CD47, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 28.9ng/ml determined by ELISA. The affinity constant of 18.9nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human CD47, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human Sirpa, hFc Tag with an affinity constant of 18.9nM as determined in SPR assay (Biacore T200).
Immobilized Human CD47, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 28.9ng/ml determined by ELISA.
The purity of Human CD47 is greater than 95% as determined by SEC-HPLC.
Human CD47 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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