Human CD83 Protein (CD8-HM183)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD83, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD83 Antibody, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Thr20-Ala143

Catalog: CD8-HM183

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Product Description Recombinant Human CD83 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr20-Ala143.

Product Background

CD83 is a member of the immunoglobulin (Ig) superfamily and is expressed in membrane bound or soluble forms. Membrane CD83 (mCD83) can be detected on a variety of activated immune cells, although it is most highly and stably expressed by mature dendritic cells (DC). While CD83 is emerging as a promising immune modulator with therapeutic potential, some important aspects such as its ligand/s, intracellular signaling pathways and modulators of its expression are unclear.

Product Category

Recombinant Protein

Target

CD83
Synonyms CD83; HB15; BL11; CD83 molecule
Accession

Species

Human

Amino Acid Range

Thr20-Ala143
Molecular Weight The protein has a predicted MW of 15.2 kDa. Due to glycosylation, the protein migrates to 18 kDa and 25-28 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human CD83, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD83 Antibody, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD83, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD83 Antibody, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA.
The purity of Human CD83 is greater than 95% as determined by SEC-HPLC.
Human CD83 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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