Human CD83 Protein (CD8-HM183)
Human CD83 Protein (CD8-HM183)
Human CD83 Protein (CD8-HM183)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human CD83, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD83 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Thr20-Ala143
Catalog #CD8-HM183
Regular price $203.00
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Product Description

Recombinant Human CD83 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr20-Ala143.

Product Background

CD83 is a member of the immunoglobulin (Ig) superfamily and is expressed in membrane bound or soluble forms. Membrane CD83 (mCD83) can be detected on a variety of activated immune cells, although it is most highly and stably expressed by mature dendritic cells (DC). While CD83 is emerging as a promising immune modulator with therapeutic potential, some important aspects such as its ligand/s, intracellular signaling pathways and modulators of its expression are unclear.

Product Category

Recombinant Protein

Protein

CD83

Synonyms

CD83; HB15; BL11; CD83 molecule

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Thr20-Ala143

Molecular Weight

The protein has a predicted MW of 15.2 kDa. Due to glycosylation, the protein migrates to 18 kDa and 25-28 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human CD83, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD83 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD83, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD83 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). Immobilized Human CD83, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD83 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).
Human CD83 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD83 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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