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Human Coagulation factor VII /F7 Protein (pro form) (CF7-HM107) Summary
Product Description |
Recombinant Human Coagulation factor VII /F7 Protein (pro form) is expressed from HEK293 with His tag at the C-terminus.It contains Ala61-Pro466. |
Product Background |
Factor VII is the coagulation protease responsible for starting a cascade of proteolytic events that lead to thrombin generation, fibrin deposition, and platelet activation. The FVII protein also contains two epidermal growth factor-like (EGF-like) domains and an activation peptide with a glycosylated asparagine that provides a proteolytic cleavage site. The catalytic regions exhibit the serine protease activity, which leads to various functions of the protein[18]. The role of FVII in the pathogenesis of various cancers has extensively been studied. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human Coagulation factor VII /F7 Protein (pro form) (CF7-HM107) Specifications/Details
Protein |
Coagulation factor VII/F7 |
Synonyms |
Proconvertin; SPCA; Eptacog alfa; F7 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala61-Pro466 |
Molecular Weight |
The protein has a predicted MW of 46.69 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result. |
Activity |
Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >80 pmol/min/µg. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 85% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 20mM NaAC, 150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in 20mM NaAC (pH 5.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
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Human Coagulation factor VII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human Coagulation factor VII is greater than 85% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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