Human Coagulation factor VII /F7 Protein (pro form) (CF7-HM107)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 85% as determined by HPLC
Activity: Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >80 pmol/min/µg.
Amino Acid Range: Ala61-Pro466

Catalog #CF7-HM107

Price

$525⁰⁰

Size

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Human Coagulation factor VII /F7 Protein (pro form) (CF7-HM107) Summary

Product Description

Recombinant Human Coagulation factor VII /F7 Protein (pro form) is expressed from HEK293 with His tag at the C-terminus.It contains Ala61-Pro466.

Product Background

Factor VII is the coagulation protease responsible for starting a cascade of proteolytic events that lead to thrombin generation, fibrin deposition, and platelet activation. The FVII protein also contains two epidermal growth factor-like (EGF-like) domains and an activation peptide with a glycosylated asparagine that provides a proteolytic cleavage site. The catalytic regions exhibit the serine protease activity, which leads to various functions of the protein[18]. The role of FVII in the pathogenesis of various cancers has extensively been studied.

Human Coagulation factor VII /F7 Protein (pro form) (CF7-HM107) Specifications/Details

Protein	Coagulation factor VII/F7
Synonyms	Proconvertin; SPCA; Eptacog alfa; F7
Accession	P08709-1
Species	Human
Biotinylated	no
Amino Acid Range	Ala61-Pro466
Molecular Weight	The protein has a predicted MW of 46.69 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result.
Activity	Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >80 pmol/min/µg.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 85% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 20mM NaAC, 150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in 20mM NaAC (pH 5.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human Coagulation factor VII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human Coagulation factor VII is greater than 85% as determined by SEC-HPLC.