• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 85% as determined by HPLC
  • Activity: Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >80 pmol/min/µg.
  • Amino Acid Range: Ala61-Pro466
Catalog #CF7-HM107
Price
Regular price $525.00
Regular price Sale price $525.00
/
Size
Avantor VWR Fisher Scientific

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Human Coagulation factor VII /F7 Protein (pro form) (CF7-HM107) Summary

Product Description

 Recombinant Human Coagulation factor VII /F7 Protein (pro form) is expressed from HEK293 with His tag at the C-terminus.It contains Ala61-Pro466.

Product Background

 Factor VII is the coagulation protease responsible for starting a cascade of proteolytic events that lead to thrombin generation, fibrin deposition, and platelet activation. The FVII protein also contains two epidermal growth factor-like (EGF-like) domains and an activation peptide with a glycosylated asparagine that provides a proteolytic cleavage site. The catalytic regions exhibit the serine protease activity, which leads to various functions of the protein[18]. The role of FVII in the pathogenesis of various cancers has extensively been studied.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human Coagulation factor VII /F7 Protein (pro form) (CF7-HM107) Specifications/Details

Protein
 Coagulation factor VII/F7
Synonyms
 Proconvertin; SPCA; Eptacog alfa; F7
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala61-Pro466
Molecular Weight
 The protein has a predicted MW of 46.69 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result.
Activity
 Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >80 pmol/min/µg.
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 85% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 20mM NaAC, 150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in 20mM NaAC (pH 5.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human Coagulation factor VII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Coagulation factor VII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Coagulation factor VII is greater than 85% as determined by SEC-HPLC. The purity of Human Coagulation factor VII is greater than 85% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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