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Human Complement Factor I Protein, Ultra Low Endotoxin (CFI-HM101-UL) Summary
Product Description |
Recombinant Human Complement Factor I Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Lys19-Val583. |
Product Background |
Complement factor I (CFI) is a serine protease which plays a key role in the modulation of complement system and the induced-fit factor responsible for controlling the complement-mediated processes. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human Complement Factor I Protein, Ultra Low Endotoxin (CFI-HM101-UL) Specifications/Details
Protein |
Complement Factor I |
Synonyms |
EC:3.4.21.45; C3B/C4B inactivator; CFI; IF; C3BINA; FIC3b-INA; I factor (complement); KAF |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Lys19-Val583 |
Molecular Weight |
The protein has a predicted MW of 64.58 kDa. Due to autocatalytic cleavage and glycosylation, the protein migrates to 40-47 kDa, 50-55 kDa and 75-95 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human Complement Factor I on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. |
|
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The purity of Human Complement Factor I is greater than 90% as determined by SEC-HPLC. |
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