Product Description |
Recombinant Human Ephrin-A4/EFNA4 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu26-Gly171. |
Product Background |
Ephrin A4 is one of the ephrin ligand molecules belonging to the tyrosine kinases receptor family. It was originally identified in a T-lymphoma cell line and seen to be expressed in human adult tissue as well as several tumor types. The cytoplasmic pattern of ephrin A4 could identify a subgroup of primary osteosarcoma patients with a high liability for progression, poor prognosis, and inferior response to chemotherapy. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
Ephrin-A4/EFNA4 |
Synonyms |
Ephrin-A4; Ephrin A4; LERK-4; EFNA4; EPLG4; EFL4 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu26-Gly171 |
Molecular Weight |
The protein has a predicted MW of 43.1 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human EphA7, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human Ephrin-A4, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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