Human EPO R/Erythropoietin R Protein (EPO-HM50R)
  • Express System: HEK293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human EPO, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human EPO R, hFc Tag with the EC50 of 35.5ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala25-Pro250
Catalog #EPO-HM50R
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Product Description
 Recombinant Human EPO R/Erythropoietin R Protein is expressed from HEK293 with hFc tag and Avi tag at the C-terminus.It contains Ala25-Pro250.
Product Background
 The erythropoietin (epo) receptor is a member of the cytokine receptor family. It is expressed almost exclusively on erythroid precursor cells and controls the development of red blood cells. The epo receptor has no intrinsic kinase activity, but binds intracellular tyrosine kinases to elicit its signals. Alterations in the transmission of the signalling cascade lead to clinically abnormal red blood cell production.
Product Category
 Recombinant Protein
Target
 EPO R/Erythropoietin R
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala25-Pro250
Molecular Weight
 The protein has a predicted MW of 52.58 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human EPO, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human EPO R, hFc Tag with the EC50 of 35.5ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-hFc-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human EPO, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human EPO R, hFc Tag with the EC50 of 35.5ng/ml determined by ELISA.
Human EPO R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human EPO R is greater than 95% as determined by SEC-HPLC.

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