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Non-biotinylated Human FGFR1 beta (IIIc) Protein (FGF-HM41C) Summary
Product Description |
Recombinant Human FGFR1 beta (IIIc) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys158-Thr355. |
Product Background |
Fibroblast growth factor receptor 1 (FGFR1) transmits signals through the plasma membrane regulating essential cellular processes like division, motility, metabolism, and death. Overexpression of FGFR1 is observed in numerous tumors and thus constitutes an attractive molecular target for selective cancer treatment. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Non-biotinylated Human FGFR1 beta (IIIc) Protein (FGF-HM41C) Specifications/Details
Protein |
FGFR1 beta (IIIc) |
Synonyms |
FGFR-1; BFGFR; bFGF-R-1; FLT-2;N-sam; CD331; |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Lys158-Thr355 |
Molecular Weight |
The protein has a predicted MW of 25.23 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Anti-FGFR1 Antibody, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human FGFR1 beta (IIIc), His Tag with the EC50 of 0.52ug/ml determined by ELISA (QC Test). |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Anti-FGFR1 Antibody, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human FGFR1 beta (IIIc), His Tag with the EC50 of 0.52ug/ml determined by ELISA (QC Test). |
|
|
Human FGFR1 beta (IIIc) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
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