Product Description |
Recombinant Human FLT3 Ligand Protein is expressed from HEK293 with hFc at the C-Terminus.It contains Thr27-Pro185. |
Product Background |
Flt‑3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. |
Product Category |
Recombinant Protein |
Target |
FLT3 Ligand |
Synonyms |
FL; Flt3 ligand; Flt3L; FLT3LG; SL cytokine |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Thr27-Pro185 |
Molecular Weight |
The protein has a predicted MW of 44.8 kDa. Due to glycosylation, the protein migrates to 90-118 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Human FLT3, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose and 0.01% tween is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human Human FLT3, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA. |
|
|
Human FLT3 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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