Human FLT3 Ligand Protein (FLT-HM23L)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human FLT3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 18.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Thr27-Pro185

Catalog: FLT-HM23L

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Product Description Recombinant Human FLT3 Ligand Protein is expressed from Expi293 with hFc at the C-terminal.It contains Thr27-Pro185.
Product Category Recombinant Protein
Target FLT3 Ligand
Synonyms FL; Flt3 ligand; Flt3L; FLT3LG; SL cytokine
Gene/Protein ID P49771-1
Species Human
Exact Sequence Thr27-Pro185
Molecular Weight The protein has a predicted MW of 44.8 kDa. Due to glycosylation, the protein migrates to 90-118 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human FLT3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 18.7ng/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose and 0.01% tween is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human FLT3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 18.7ng/ml determined by ELISA.
Human FLT3 Ligand on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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