ELISA binding curve of Human FLT3 Ligand with hFc tag showing OD450 response to antibody concentration.
Human FLT3 Ligand Protein (FLT-HM23L)
Human FLT3 Ligand Protein (FLT-HM23L)
Human FLT3 Ligand Protein (FLT-HM23L)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human FLT3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 35.7ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Thr27-Pro185
Catalog #FLT-HM23L
Regular price $363.00
/
Size

Order by 4pm ET for delivery on:

Human FLT3 Ligand Protein (FLT-HM23L) Summary

Product Description

Recombinant Human FLT3 Ligand Protein is expressed from HEK293 with hFc at the C-Terminus.It contains Thr27-Pro185.

Product Background

Flt‑3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human FLT3 Ligand Protein (FLT-HM23L) Specifications/Details

Protein
FLT3 Ligand
Synonyms
FL; Flt3 ligand; Flt3L; FLT3LG; SL cytokine; Flt-3 Ligand; Flt-3L
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Thr27-Pro185
Molecular Weight
The protein has a predicted MW of 44.8 kDa. Due to glycosylation, the protein migrates to 90-118 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human FLT3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 35.7ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-hFc
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose and 0.01% tween is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human FLT3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 35.7ng/ml determined by ELISA. Immobilized Human FLT3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 35.7ng/ml determined by ELISA.
Human FLT3 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human FLT3 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human FLT3 Ligand is greater than 95% as determined by SEC-HPLC. The purity of Human FLT3 Ligand is greater than 95% as determined by SEC-HPLC.
Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Recently viewed