Human FLT3 Ligand Protein (FLT-HM23L)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human Human FLT3, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Thr27-Pro185

Catalog: FLT-HM23L

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Product Description	Recombinant Human FLT3 Ligand Protein is expressed from HEK293 with hFc at the C-Terminus.It contains Thr27-Pro185.
Product Background	Flt‑3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
Product Category	Recombinant Protein

Target	FLT3 Ligand
Synonyms	FL; Flt3 ligand; Flt3L; FLT3LG; SL cytokine
Gene/Protein ID	P49771-1
Species	Human
Exact Sequence	Thr27-Pro185
Molecular Weight	The protein has a predicted MW of 44.8 kDa. Due to glycosylation, the protein migrates to 90-118 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human Human FLT3, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose and 0.01% tween is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human Human FLT3, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA.
	Human FLT3 Ligand on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.