Human GDNF Protein (GDF-HE001)
Catalog: GDF-HE001
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Product Description | Recombinant Human GDNF Protein is expressed from E.coli without tagIt contains Ser78-Ile211. |
Product Background |
Glial cell line-derived neurotrophic factor (GDNF) is a 134 amino acid protein belonging in the GDNF family ligands (GFLs). GDNF was originally isolated from rat glial cell lines and identified as a neurotrophic factor with the ability to promote dopamine uptake within midbrain dopaminergic neurons. |
Product Category |
Recombinant Protein |
Target |
GDNF |
Accession | |
Species |
Human |
Amino Acid Range |
Ser78-Ile211 |
Molecular Weight | The protein has a predicted MW of 15.06 kDa same as Tris-Bis PAGE result. |
Activity |
Immobilized Human GDNF, No Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.8ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
No Tag |
Express System |
E.coli |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 0.1 EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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