ELISA binding assay showing dose-dependent interaction between Human GDNF protein (no tag) and Human GFR alpha 1, His Tag.
Human GDNF Protein (GDF-HE001)
Human GDNF Protein (GDF-HE001)
Human GDNF Protein (GDF-HE001)
Human GDNF Protein (GDF-HE001)
  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human GDNF, No Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ser78-Ile211
Catalog #GDF-HE001
Regular price $379.50
/
Size

Order by 4pm ET for delivery on:

Human GDNF Protein (GDF-HE001) Summary

Product Description

Recombinant Human GDNF Protein is expressed from E.coli without tagIt contains Ser78-Ile211.

Product Background

Glial cell line-derived neurotrophic factor (GDNF) is a 134 amino acid protein belonging in the GDNF family ligands (GFLs). GDNF was originally isolated from rat glial cell lines and identified as a neurotrophic factor with the ability to promote dopamine uptake within midbrain dopaminergic neurons.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human GDNF Protein (GDF-HE001) Specifications/Details

Protein
GDNF
Synonyms
Glial cell line-derived neurotrophic factor; hGDNF; Astrocyte-derived trophic factor (ATF)
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ser78-Ile211
Molecular Weight
The protein has a predicted MW of 15.06 kDa same as Bis-Tris PAGE result.
Activity
Immobilized Human GDNF, No Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
No Tag
Expression System
E.coli
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human GDNF, No Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). Immobilized Human GDNF, No Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).
Human GDNF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human GDNF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50-for this effect is 2‑12 ng/mL in the presence of Recombinant Human GFR alpha ‑1/GDNF-R alpha ‑1 Fc-Chimera. Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50-for this effect is 2‑12 ng/mL in the presence of Recombinant Human GFR alpha ‑1/GDNF-R alpha ‑1 Fc-Chimera.
The purity of Human GDNF is greater than 95% as determined by SEC-HPLC. The purity of Human GDNF is greater than 95% as determined by SEC-HPLC.
Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Recently viewed