Product Description |
Recombinant Human GDNF Protein is expressed from E.coli without tagIt contains Ser78-Ile211. |
Product Background |
Glial cell line-derived neurotrophic factor (GDNF) is a 134 amino acid protein belonging in the GDNF family ligands (GFLs). GDNF was originally isolated from rat glial cell lines and identified as a neurotrophic factor with the ability to promote dopamine uptake within midbrain dopaminergic neurons. |
Product Category |
Recombinant Protein |
Target |
GDNF |
Synonyms |
Glial cell line-derived neurotrophic factor; hGDNF; Astrocyte-derived trophic factor (ATF) |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser78-Ile211 |
Molecular Weight |
The protein has a predicted MW of 15.06 kDa same as Bis-Tris PAGE result. |
Activity |
Immobilized Human GDNF, No Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.8ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
No Tag |
Express System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human GDNF, No Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.8ng/ml determined by ELISA. |
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Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50-for this effect is 2‑12 ng/mL in the presence of Recombinant Human GFR alpha ‑1/GDNF-R alpha ‑1 Fc-Chimera. |
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Human GDNF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human GDNF is greater than 95% as determined by SEC-HPLC. |
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