Human GDNF Protein (GDF-HE001)

  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human GDNF, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 5.0ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ser78-Ile211

Catalog: GDF-HE001

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Product Description Recombinant Human GDNF Protein is expressed from E.coli without tagIt contains Ser78-Ile211.

Product Background

Glial cell line-derived neurotrophic factor (GDNF) is a 134 amino acid protein belonging in the GDNF family ligands (GFLs). GDNF was originally isolated from rat glial cell lines and identified as a neurotrophic factor with the ability to promote dopamine uptake within midbrain dopaminergic neurons.

Product Category

Recombinant Protein

Target

GDNF

Gene/Protein ID

P39905-1

Species

Human

Exact Sequence

Ser78-Ile211
Molecular Weight The protein has a predicted MW of 15.06 kDa same as Tris-Bis PAGE result.

Activity

Immobilized Human GDNF, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 5.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

No Tag

Express System

E.coli
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human GDNF, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 5.0ng/ml determined by ELISA.
The purity of Human GDNF is greater than 95% as determined by SEC-HPLC.
Human GDNF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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