Human GDNF Protein (GDF-HE001)

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human GDNF, No Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.8ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Ser78-Ile211

Catalog: GDF-HE001

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Product Description	Recombinant Human GDNF Protein is expressed from E.coli without tagIt contains Ser78-Ile211.
Product Background	Glial cell line-derived neurotrophic factor (GDNF) is a 134 amino acid protein belonging in the GDNF family ligands (GFLs). GDNF was originally isolated from rat glial cell lines and identified as a neurotrophic factor with the ability to promote dopamine uptake within midbrain dopaminergic neurons.
Product Category	Recombinant Protein

Target	GDNF
Accession	P39905-1
Species	Human
Amino Acid Range	Ser78-Ile211
Molecular Weight	The protein has a predicted MW of 15.06 kDa same as Tris-Bis PAGE result.
Activity	Immobilized Human GDNF, No Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	No Tag
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human GDNF, No Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.8ng/ml determined by ELISA.
	The purity of Human GDNF is greater than 95% as determined by SEC-HPLC.
	Human GDNF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.