Human GDNF Protein (GDF-HE001)

  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human GDNF, No Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ser78-Ile211

Catalog: GDF-HE001

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Product Description Recombinant Human GDNF Protein is expressed from E.coli without tagIt contains Ser78-Ile211.
Product Category Recombinant Protein
Target GDNF
Gene/Protein ID P39905-1
Species Human
Exact Sequence Ser78-Ile211
Molecular Weight The protein has a predicted MW of 15.06 kDa same as Tris-Bis PAGE result.
Activity Immobilized Human GDNF, No Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.7ng/ml determined by ELISA. See testing image for detail.
Product Tag No Tag
Express System E.coli
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human GDNF, No Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.7ng/ml determined by ELISA.
The purity of Human GDNF is greater than 95% as determined by SEC-HPLC.
Human GDNF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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