Name: Human GITR Ligand/TNFSF18 Trimer Protein (FSF-HM418)
Brand: KactusBio
SKU: FSF-HM418-100UG
Price: 270.0 USD
Availability: InStock

Human GITR Ligand/TNFSF18 Trimer Protein (FSF-HM418)

Express System: HEK293
Product Tag: N-His-Flag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human GITR Ligand Trimer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human GITR, hFc Tag with the EC50 of 50.8ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gln50-Ser177

Catalog #FSF-HM418

Size

100UG

500UG

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Product Description	Recombinant Human GITR Ligand/TNFSF18 Trimer Protein is expressed from HEK293 with His tag and flag tag at the N-Terminus.It contains Gln50-Ser177.
Product Background	Glucocorticoid-induced TNFR-related protein (TNFRSF18, GITR, CD357), expressed by T cells, and its ligand (TNFSF18, GITRL), expressed by myeloid populations, provide co-stimulatory signals that boost T cell activity. Due to the important role that GITR plays in regulating immune functions, agonistic stimulation of GITR is a promising therapeutic concept.
Product Category	Recombinant Protein

Target	GITR Ligand/TNFSF18 Trimer
Synonyms	TNFSF18; AITRL; TL6; GITRL; GITR Ligand
Accession	Q9UNG2
Species	Human
Biotinylated	no
Amino Acid Range	Gln50-Ser177
Molecular Weight	The protein has a predicted MW of 47.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human GITR Ligand Trimer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human GITR, hFc Tag with the EC50 of 50.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His-Flag
Express System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human GITR Ligand Trimer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human GITR, hFc Tag with the EC50 of 50.8ng/ml determined by ELISA.
	Human GITR Ligand Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human GITR Ligand Trimer is greater than 95% as determined by SEC-HPLC.

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