Human GITR Ligand/TNFSF18 Trimer Protein (FSF-HM418) Summary
Product Description |
Recombinant Human GITR Ligand/TNFSF18 Trimer Protein is expressed from HEK293 with His tag and flag tag at the N-Terminus.It contains Gln50-Ser177 trimer design. |
Product Background |
Glucocorticoid-induced TNFR-related protein (TNFRSF18, GITR, CD357), expressed by T cells, and its ligand (TNFSF18, GITRL), expressed by myeloid populations, provide co-stimulatory signals that boost T cell activity. Due to the important role that GITR plays in regulating immune functions, agonistic stimulation of GITR is a promising therapeutic concept. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Human GITR Ligand/TNFSF18 Trimer Protein (FSF-HM418) Specifications/Details
Protein |
GITR Ligand/TNFSF18 |
Synonyms |
TNFSF18; AITRL; TL6; GITRL; GITR Ligand |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln50-Ser177 |
Molecular Weight |
The protein has a predicted MW of 47.5 kDa. Due to glycosylation, the protein migrates to 54-64 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human GITR Ligand Trimer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human GITR, hFc Tag with the EC50 of 50.8ng/ml determined by ELISA. |
Product Tag |
N-His-Flag |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human GITR Ligand Trimer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human GITR, hFc Tag with the EC50 of 50.8ng/ml determined by ELISA. |
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Human GITR Ligand Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human GITR Ligand Trimer is greater than 95% as determined by SEC-HPLC. |
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