Product Description |
Recombinant Human GPA34/VSIG1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val22-Glu232. |
Product Background |
VSIG1, a cell adhesion protein of the immunoglobulin superfamily, is preferentially expressed in stomach, testis, and certain gastric, esophageal and ovarian cancers. Here, we describe the expression patterns of three alternatively spliced isoforms of mouse Vsig1 during pre- and postnatal development of stomach and potential function of Vsig1 in differentiation of gastric epithelia. VSIG1 is required for the establishment of glandular versus squamous epithelia in the stomach. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
GPA34/VSIG1 |
Synonyms |
Glycoprotein A34; Gpa34; dJ889N15.1; MGC44287,1700062D20Rik; VSIG1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Val22-Glu232 |
Molecular Weight |
The protein has a predicted MW of 24 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Human GPA34 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human GPA34 is greater than 95% as determined by SEC-HPLC. |
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