Name: Human HGF R/c-MET Protein (MET-HM201)
Brand: KactusBio
SKU: MET-HM201-100UG
Price: 214.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human HGF, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human HGF R, hFc Tag with the EC50 of 9.9 ng/ml determined by ELISA (QC Test).
Amino Acid Range: Glu25-Thr932

Catalog #MET-HM201

Size

100UG

500UG

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Human HGF R/c-MET Protein (MET-HM201) Summary

Product Description	Recombinant Human HGF R/c-MET Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Glu25-Thr932.
Product Background	c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGF R), is a protein that in humans is encoded by the MET gene. The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling.
Product Category	Recombinant Protein / Antibody discovery / ADC targets

Human HGF R/c-MET Protein (MET-HM201) Specifications/Details

Protein	HGF R/c-MET
Synonyms	MET; oncogene MET; HGF R; HGF/SF receptor; AUTS9; cMET; Met (c-Met); RCCP2; SF receptor
Accession	P08581-1
Species	Human
Biotinylated	no
Amino Acid Range	Glu25-Thr932
Molecular Weight	The mature form of HGF R is a heterodimer which can be cleaved into α and β chain. The protein has a predicted MW of 32.5 kDa (α chain) and 95.9 kDa (β chain Fc chimera). Due to glycosylation, the protein migrates to 45-55 kDa and 100-120 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human HGF, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human HGF R, hFc Tag with the EC50 of 9.9 ng/ml determined by ELISA (QC Test).
Product Tag	C-hFc (IgG1)
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human HGF, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human HGF R, hFc Tag with the EC50 of 9.9 ng/ml determined by ELISA (QC Test).
	Immobilized Human HGF R, hFc Tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-HGF R Antibody, hFc Tag with the EC50 of 4.2 ng/ml determined by ELISA.
	Human HGF R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Human HGF R, hFc Tag on ProA-Biosensor can bind Human HGF, His Tag with an affinity constant of 4.32 nM as determined in BLI assay (Gator® Prime).
	The purity of Human HGF R is greater than 90% as determined by SEC-HPLC.