Product Description |
Recombinant Human HMGB1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met1-Glu215. |
Product Background |
High-mobility group box 1 (HMGB1) is a ubiquitous nuclear protein that promotes inflammation when released extracellularly after cellular activation, stress, damage or death. HMGB1 operates as one of the most intriguing molecules in inflammatory disorders via recently elucidated signal and molecular transport mechanisms. Treatments based on antagonists specifically targeting extracellular HMGB1 have generated encouraging results in a wide number of experimental models of infectious and sterile inflammation. |
Product Category |
Recombinant Protein |
Target |
HMGB1 |
Synonyms |
HMGB1; HMG1; HMG3; SBP-1; HMG-1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met1-Glu215 |
Molecular Weight |
The protein has a predicted MW of 26 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human HMGB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human HMGB1 is greater than 95% as determined by SEC-HPLC. |
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