Human ICOS/CD278 Protein (ICO-HM101)
Human ICOS/CD278 Protein (ICO-HM101)
Human ICOS/CD278 Protein (ICO-HM101)
Human ICOS/CD278 Protein (ICO-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human ICOS, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 25.3ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Glu21-Gln134
Catalog #ICO-HM101
Regular price $315.00
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Product Description
Recombinant Human ICOS/CD278 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu21-Gln134.
Product Background
Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain.
Product Category
Recombinant Protein
Target
ICOS/CD278
Synonyms
AILIM; CD278; CRP-1; CVID1; ICOS; MGC39850
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Glu21-Gln134
Molecular Weight
The protein has a predicted MW of 14.6 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human ICOS, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 25.3ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human ICOS, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 25.3ng/ml determined by ELISA.
Human ICOS on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
The purity of Human ICOS is greater than 90% as determined by SEC-HPLC.

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