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Non-biotinylated Human IL-15RA/IL-15 R alpha/CD215 Protein (IL5-HM4RA) Summary
Product Description |
Recombinant Human IL-15RA/IL-15 R alpha/CD215 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile31-Thr205. |
Product Background |
Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Non-biotinylated Human IL-15RA/IL-15 R alpha/CD215 Protein (IL5-HM4RA) Specifications/Details
Protein |
IL-15RA/IL-15 R alpha/CD215 |
Synonyms |
IL-15 R alpha; CD215; IL-15RA; MGC104179 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ile31-Thr205 |
Molecular Weight |
The protein has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-15 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-15RA, His Tag with the EC50 of 15.4ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Human IL-15 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-15RA, His Tag with the EC50 of 15.4ng/ml determined by ELISA. |
|
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Human IL-15RA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human IL-15RA is greater than 95% as determined by SEC-HPLC. |
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