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Non-biotinylated Human IL-2 R beta/CD122 Protein (CD1-HM422) Summary
Product Description |
Recombinant Human IL-2 R beta/CD122 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Asp239. |
Product Background |
IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid residue precursor Type I membrane protein with a 26 residue signal peptide, a 214 residue extracellular region, a 25 residue transmembrane region and a 286 residue cytoplasmic domain. Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains, and the intermediate affinity complex comprising heterodimers of the beta and gamma chains. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Non-biotinylated Human IL-2 R beta/CD122 Protein (CD1-HM422) Specifications/Details
Protein |
IL-2 R beta/CD122 |
Synonyms |
CD122; IL-15 R beta; IL-2 R beta; IL-2R subunit beta; IL2RB; P70-75; p75; RP5-1170K4.6; IL-15 R β; IL-2 R β; IL-2R subunit β |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala27-Asp239 |
Molecular Weight |
The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-2 R beta, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-15Ra&IL-15, hFc Tag with the EC50 of 43.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.12 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human IL-2 R beta, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-2, No Tag with an affinity constant of 0.12 uM as determined in SPR assay (Biacore T200). |
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Human IL-2 R beta, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-15, No Tag with an affinity constant of 5.9nM as determined in SPR assay (Biacore T200). |
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Immobilized Human IL-2 R beta, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-15Ra&IL-15, hFc Tag with the EC50 of 43.7ng/ml determined by ELISA (QC Test). |
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Human IL-2 R beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human IL-2 R beta is greater than 95% as determined by SEC-HPLC. |
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