Human Kallikrein 4/KLK4 Protein (active form), Ultra Low Endotoxin (KL

Express System: HEK293
Product Tag: C-His
Purity: > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >1200 pmol/min/µg.
Amino Acid Range: Ile31-Ser254

Catalog #KLK-HM104-UL

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$454.00

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Human Kallikrein 4/KLK4 Protein (active form), Ultra Low Endotoxin (KLK-HM104-UL) Summary

Product Description	Recombinant Human Kallikrein 4/KLK4 Protein (active form) is expressed from HEK293 with His tag at the C-terminus.It contains Ile31-Ser254.
Product Background	Human tissue kallikrein 4 (hK4) is a member of a highly conserved gene family which contains 15 human enzymes composed of closely related serine proteases. The hK4 enzyme is a subgroup of the serine proteases that catalyzes the cutting of the peptide backbone in proteins via hydrolysis. kallikreins been known to catalyze the break down of bradykininogen to bradykinin and plasminogen to plasmin, in addition to being active in initiating intracellular signaling through protease-activated receptors.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	Kallikrein 4/KLK4
Synonyms	Kallikrein-4; KLK-L1; Prostase; KLK4; EMSP1; PRSS17; PSTS
Accession	Q9Y5K2-1
Species	Human
Biotinylated	no
Amino Acid Range	Ile31-Ser254
Molecular Weight	The protein has a predicted MW of 25.61 kDa. Due to glycosylation, the protein migrates to 27-37 kDa based on Bis-Tris PAGE result.
Activity	Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >1200 pmol/min/µg.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 20mM HEPES, 150mM NaCl, 8% trehalose, 0.05% Brij-35, 5mM Benzamidine (pH 7.5).
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human Kallikrein 4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human Kallikrein 4 is greater than 95% as determined by SEC-HPLC.

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