Human Kallikrein 4/KLK4 Protein (active form), Ultra Low Endotoxin (KLK-HM104-UL)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >1200 pmol/min/µg.
  • Amino Acid Range: Ile31-Ser254
  • Other Available Proteins:
    Loading...
Catalog #KLK-HM104-UL
/
Size

Order by 4pm ET for delivery on:

Human Kallikrein 4/KLK4 Protein (active form), Ultra Low Endotoxin (KLK-HM104-UL) Summary

Product Description

 Recombinant Human Kallikrein 4/KLK4 Protein (active form) is expressed from HEK293 with His tag at the C-terminus.It contains Ile31-Ser254.

Product Background

 Human tissue kallikrein 4 (hK4) is a member of a highly conserved gene family which contains 15 human enzymes composed of closely related serine proteases. The hK4 enzyme is a subgroup of the serine proteases that catalyzes the cutting of the peptide backbone in proteins via hydrolysis. kallikreins been known to catalyze the break down of bradykininogen to bradykinin and plasminogen to plasmin, in addition to being active in initiating intracellular signaling through protease-activated receptors.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human Kallikrein 4/KLK4 Protein (active form), Ultra Low Endotoxin (KLK-HM104-UL) Specifications/Details

Protein
 Kallikrein 4/KLK4
Synonyms
 Kallikrein-4; KLK-L1; Prostase; KLK4; EMSP1; PRSS17; PSTS
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ile31-Ser254
Molecular Weight
 The protein has a predicted MW of 25.61 kDa. Due to glycosylation, the protein migrates to 27-37 kDa based on Bis-Tris PAGE result.
Activity
 Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >1200 pmol/min/µg.
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 20mM HEPES, 150mM NaCl, 8% trehalose, 0.05% Brij-35, 5mM Benzamidine (pH 7.5).
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human Kallikrein 4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Kallikrein 4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Kallikrein 4 is greater than 95% as determined by SEC-HPLC. The purity of Human Kallikrein 4 is greater than 95% as determined by SEC-HPLC.
Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Contact Us

support@kactusbio.us

Recommended Products

Recently viewed