Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer Protein (MHC-HM418)
Catalog: MHC-HM418
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| Product Description | Recombinant Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*03:01),Ile21-Met119(B2M) and VVVGAVGVGK peptide. |
| Product Category | Recombinant Protein |
| Target | HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer |
| Synonyms | MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD |
| Gene/Protein ID | NP_002107.3(HLA-A*03:01)&P61769(B2M)&VVVGAVGVGK |
| Species | Human |
| Exact Sequence | Gly25-Thr305(HLA-A*03:01),Ile21-Met119(B2M)and VVVGAVGVGK peptide |
| Molecular Weight | The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result. |
| Activity | The affinity constant of 0.11 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer, His Tag captured on CM5 Chip via anti-his antibody can bind Anti-HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Antibody with an affinity constant of 0.11 uM as determined in SPR assay (Biacore T200). |
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The purity of Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer is greater than 95% as determined by SEC-HPLC. |
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Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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