Name: Human LGR-5 Protein (LGR-HM205)
Brand: KactusBio
SKU: LGR-HM205-100UG
Price: 346.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc (IgG1)
Purity: > 90% as determined by Bis-Tris PAGE
Activity: The affinity constant of 0.59 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Gly22-Pro543
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Catalog #LGR-HM205

Size

100UG

500UG

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Human LGR-5 Protein (LGR-HM205) Summary

Product Description	Recombinant Human LGR-5 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gly22-Pro543.
Product Background	G protein-coupled receptor 5 (LGR5), known as a stem cell marker for colon cancer and gastric cancer, can serve as a novel GSC marker involved in EMT and a therapeutic target in glioma.LGR5 is a new functional GSC marker and prognostic indicator that can promote EMT by activating the Wnt/β-catenin pathway and would thus be a novel therapeutic target for glioma.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human LGR-5 Protein (LGR-HM205) Specifications/Details

Protein	LGR-5
Synonyms	LGR5; GPR49; GPR67; HG38; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38
Accession	O75473-1
Species	Human
Biotinylated	no
Amino Acid Range	Gly22-Pro543
Molecular Weight	The protein has a predicted MW of 85 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 0.59 nM as determined in SPR assay (Biacore T200).
Product Tag	C-hFc (IgG1)
Expression System	HEK293
Purity	> 90% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS, 5mM DTT (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human LGR-5, hFc Tag captured on CM5 Chip via Protein A can bind Human R-Spondin 3, His Tag with an affinity constant of 0.59 nM as determined in SPR assay (Biacore T200).
	Human LGR-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.