Human LGR-5 Protein (LGR-HM205)
Catalog: LGR-HM205
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| Product Description | Recombinant Human LGR-5 Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Gly22-Pro543. |
| Product Category | Recombinant Protein |
| Target | LGR-5 |
| Gene/Protein ID | O75473-1 |
| Species | Human |
| Exact Sequence | Gly22-Pro543 |
| Molecular Weight | The protein has a predicted MW of 85 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result. |
| Activity | The affinity constant of 0.59 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-hFc |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Liquid |
| Shipping | Shipped with blue ice. |
| Formulation | Supplied as 0.22μm filtered solution in 20mM PB, 300mM NaCl, 5mM DTT, 100mM L-Arginine (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label. |
| Stability And Storage | Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles. |
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Human LGR-5, hFc Tag captured on CM5 Chip via Protein A can bind Human R-Spondin 3, His Tag with an affinity constant of 0.59 nM as determined in SPR assay (Biacore T200). |
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The purity of Human LGR-5 is greater than 95% as determined by SEC-HPLC. |
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Human LGR-5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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