Product Description |
Recombinant Human LGR-5 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly22-Pro543. |
Product Background |
G protein-coupled receptor 5 (LGR5), known as a stem cell marker for colon cancer and gastric cancer, can serve as a novel GSC marker involved in EMT and a therapeutic target in glioma.LGR5 is a new functional GSC marker and prognostic indicator that can promote EMT by activating the Wnt/β-catenin pathway and would thus be a novel therapeutic target for glioma. |
Product Category |
Recombinant Protein |
Target |
LGR-5 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly22-Pro543 |
Molecular Weight |
The protein has a predicted MW of 85 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.59 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS, 5mM DTT (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human LGR-5, hFc Tag captured on CM5 Chip via Protein A can bind Human R-Spondin 3, His Tag with an affinity constant of 0.59 nM as determined in SPR assay (Biacore T200). |
|
|
Human LGR-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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