Human LGR-5 Protein (LGR-HM205)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE
Activity: The affinity constant of 0.59 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gly22-Pro543

Catalog: LGR-HM205

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Product Description	Recombinant Human LGR-5 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly22-Pro543.
Product Background	G protein-coupled receptor 5 (LGR5), known as a stem cell marker for colon cancer and gastric cancer, can serve as a novel GSC marker involved in EMT and a therapeutic target in glioma.LGR5 is a new functional GSC marker and prognostic indicator that can promote EMT by activating the Wnt/β-catenin pathway and would thus be a novel therapeutic target for glioma.
Product Category	Recombinant Protein

Target	LGR-5
Gene/Protein ID	O75473-1
Species	Human
Exact Sequence	Gly22-Pro543
Molecular Weight	The protein has a predicted MW of 85 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 0.59 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS, 5mM DTT (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human LGR-5, hFc Tag captured on CM5 Chip via Protein A can bind Human R-Spondin 3, His Tag with an affinity constant of 0.59 nM as determined in SPR assay (Biacore T200).
	Human LGR-5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.