Name: Human LGR-5 Protein (LGR-HM205)
Brand: KactusBio
SKU: LGR-HM205-100UG
Price: 315.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE
Activity: The affinity constant of 0.59 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gly22-Pro543

Catalog #LGR-HM205

Size

100UG

500UG

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Product Description	Recombinant Human LGR-5 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly22-Pro543.
Product Background	G protein-coupled receptor 5 (LGR5), known as a stem cell marker for colon cancer and gastric cancer, can serve as a novel GSC marker involved in EMT and a therapeutic target in glioma.LGR5 is a new functional GSC marker and prognostic indicator that can promote EMT by activating the Wnt/β-catenin pathway and would thus be a novel therapeutic target for glioma.
Product Category	Recombinant Protein / Antibody discovery

Protein	LGR-5
Synonyms	LGR5; GPR49; GPR67; HG38; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38
Accession	O75473-1
Species	Human
Biotinylated	no
Amino Acid Range	Gly22-Pro543
Molecular Weight	The protein has a predicted MW of 85 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 0.59 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS, 5mM DTT (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human LGR-5, hFc Tag captured on CM5 Chip via Protein A can bind Human R-Spondin 3, His Tag with an affinity constant of 0.59 nM as determined in SPR assay (Biacore T200).
	Human LGR-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.