Human LY75/CD205 Protein (LY7-HM105)

SKU: LY7-HM105-100UG

Human LY75/CD205 Protein (LY7-HM105)

SKU: LY7-HM105-100UG
ELISA binding curve of human LY75 His tag protein with anti-LY75 hFc-tagged antibody, showing dose-dependent absorbance at OD450.
Human LY75/CD205 Protein (LY7-HM105)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human LY75, His Tag at 0.1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LY75 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.
  • Amino Acid Range: Ser28-Asp1666
Catalog #LY7-HM105
Regular price $330.00
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Size
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Human LY75/CD205 Protein (LY7-HM105) Summary

Product Description

Recombinant Human LY75/CD205 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser28-Asp1666.

Product Background

Lymphocyte antigen 75 (Ly75/CD205) is a surface marker on mitotic germ cells in rainbow trout. In mammals, several cell surface molecular markers have been characterized in order to identify the mitotic germ cells. he expression profile of Ly75 protein was similar to that of the mRNA. Furthermore, identification of various fish homologs of ly75 confirmed that their amino acid sequences are well conserved. Therefore, Ly75 may be appropriate for use as a versatile cell surface marker for mitotic germ cells in fish.

Product Category

Recombinant Protein / Antibody discovery / ADC targets

Human LY75/CD205 Protein (LY7-HM105) Specifications/Details

Protein
LY75/CD205
Synonyms
Ly-75; DEC-205; CD205; CLEC13B; GP200-MR6
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ser28-Asp1666
Molecular Weight
The protein has a predicted MW of 190.2 kDa. Due to glycosylation, the protein migrates to 230-245 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human LY75, His Tag at 0.1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LY75 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.
Product Tag
C-His

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human LY75, His Tag at 0.1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LY75 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. Immobilized Human LY75, His Tag at 0.1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LY75 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.
Human LY75 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human LY75 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...
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