Human LY75/CD205 Protein (LY7-HM105)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human LY75, His Tag at 0.1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LY75 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ser28-Asp1666

Catalog: LY7-HM105

Order now get it on

Bulk Quote | Test Sample

Product Description	Recombinant Human LY75/CD205 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser28-Asp1666.
Product Background	Lymphocyte antigen 75 (Ly75/CD205) is a surface marker on mitotic germ cells in rainbow trout. In mammals, several cell surface molecular markers have been characterized in order to identify the mitotic germ cells. he expression profile of Ly75 protein was similar to that of the mRNA. Furthermore, identification of various fish homologs of ly75 confirmed that their amino acid sequences are well conserved. Therefore, Ly75 may be appropriate for use as a versatile cell surface marker for mitotic germ cells in fish.
Product Category	Recombinant Protein

Target	LY75/CD205
Synonyms	Ly-75; DEC-205; CD205; CLEC13B; GP200-MR6
Gene/Protein ID	O60449-1
Species	Human
Exact Sequence	Ser28-Asp1666
Molecular Weight	The protein has a predicted MW of 190.2 kDa. Due to glycosylation, the protein migrates to 230-245 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human LY75, His Tag at 0.1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LY75 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human LY75, His Tag at 0.1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LY75 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.
	The purity of Human LY75 is greater than 95% as determined by SEC-HPLC.
	Human LY75 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.