Human LY75/CD205 Protein (LY7-HM105)
Human LY75/CD205 Protein (LY7-HM105)
Human LY75/CD205 Protein (LY7-HM105)
Human LY75/CD205 Protein (LY7-HM105)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LY75, His Tag at 0.1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LY75 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ser28-Asp1666
Catalog #LY7-HM105
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Product Description
 Recombinant Human LY75/CD205 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser28-Asp1666.
Product Background
 Lymphocyte antigen 75 (Ly75/CD205) is a surface marker on mitotic germ cells in rainbow trout. In mammals, several cell surface molecular markers have been characterized in order to identify the mitotic germ cells. he expression profile of Ly75 protein was similar to that of the mRNA. Furthermore, identification of various fish homologs of ly75 confirmed that their amino acid sequences are well conserved. Therefore, Ly75 may be appropriate for use as a versatile cell surface marker for mitotic germ cells in fish.
Product Category
 Recombinant Protein
Target
 LY75/CD205
Synonyms
 Ly-75; DEC-205; CD205; CLEC13B; GP200-MR6
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ser28-Asp1666
Molecular Weight
 The protein has a predicted MW of 190.2 kDa. Due to glycosylation, the protein migrates to 230-245 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human LY75, His Tag at 0.1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LY75 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human LY75, His Tag at 0.1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LY75 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.
Human LY75 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human LY75 is greater than 95% as determined by SEC-HPLC.
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